Biofilm Treatment


Biofilms are basically slimy films containing mucous and minerals that form a protective layer over bacteria and fungi. They’re common in nature. This is why rocks in a stream are slippery and there’s scum on your teeth, etc. The problem is in the gastrointestinal tract; the biofilms can protect bacterial pathogens, which excrete toxins.

Toxic biofilms are not just a problem for autistic children but also for adults suffering from a variety of chronic illnesses. The bacteria and fungi within biofilms are very difficult to eradicate because layers of mucous and minerals in the biofilm protect them. Chronic toxic biofilms are the reason many children are resistant to treatment or hit plateaus. The surface bacteria receive treatment but not those hidden within the biofilms. In order to get at these protected organisms, you have to disrupt the biofilm and establish a new biofilm with healthy flora.

So, how do we treat biofilms. It’s generally a three step process.

1. Break down the biofilms with the use of enzymes and chelators.

2. Add in antibacterial and/or antifungal agents; these are in the form of herbal preparations or medications.

3. Clean up the dead bacteria and fungi with binding agents.

What you need

1. Enzymes: You need an enzyme product to break down the biofilm. You only need one of the recommended enzyme products, which are Interfase, Interfase Plus, SPS30 or Lubrokinase 20mg capsules, Nattokinase 50mg to 100 mg capsules. (Children under 80 pounds use 50mg.) We generally recommend the Interfase Plus because it also contains the chelating agent.

2. Chelator: Either EDTA capsules (not tablets) or Interfase Plus, which is a combination enzyme with chelators added.

3. Antibacterial/Antifungal Agents: ProEnt2 capsules, ADP (not suitable for small children or those who don’t swallow pills since the taste is very strong), olive leaf extract, grape seed extract, Biocidin or similar product.

You can also use medications with this protocol, but they require a prescription. If your child is already on an antibiotic or antifungal, you may find they’re more effective when used with the other steps in the protocol. Flagyl, Vancomycin, and/or antifungals such as Diflucan, Nystatin, and amphotericin are medications commonly prescribed to treat digestive tract dysbiosis (microbial imbalance).

The rest of this protocol contains items that you may or may not require to control die-off reactions.

4. Binding Agents: We recommend combination products such as Elmnx or ExTox, but a variety of agents are useful such as modified fruit pectin, activated charcoal, Tru Fiber, Metal Magnet, etc.

5. Protective Agents: Oxyperm is an antioxidant made for the digestive system and is our recommended product. Give it with meals. Oxyperm will help reduce reactions that can occur. Give Oxyperm three times a day with meals.


Enzymes: We use Interfase Plus or SPS30 alone or Lumbrokinase and Nattokinase in combination. Interfase Plus is the most common because it also contains a chelating agent. Do not give enzymes on an empty stomach along with a chelating agent to bind up the heavy metals and other minerals found in the biofilm. It’s essential that the enzymes are NOT given with food because they will break down just the food and not the biofilm.

Chelating Agent: Give the chelating agent with the enzymes. Interfase Plus is a combination of enzymes and chelators and generally works best.

Wait 30 to 60 minutes for the enzymes and chelators to work.

Killing Agents: Add the antifungal and/or antibiotic herbs or medications. These are generally given with meals to reduce problems with stomach irritation along with Oxyperm to reduce adverse effects.

Binding Agents: Give Elmnx (3 to 6 capsules) or Extox (4 to 6 capsules) and Tru Fiber an hour or more after the killing agents. Binding agents are often given at bedtime. Binding agents will bind and prevent absorption of nutrients, so give binding agents at least one hour before or after other medicines or supplements.

Probiotics: Probiotics help rebuild and restore normal flora. The best time to give them is on an empty stomach once or twice daily.

Duration: Follow the biofilm protocol for four to six months.

Notes: You may see very abnormal-looking stools when on this protocol; this is expected. You also may see pretty strong Herxheimer reactions. A Herxheimer reaction is a flu-like reaction that occurs when the protocols kill the fungus or bacterial in high numbers and release their toxins. See The protective agents may prevent or reduce the severity of Herxheimer reactions. If the Herxheimer reactions are very intense, you may need to reduce the dose of the killing agents and increase the dose of the binding agents. We often substitute high-dose probiotics for the killing agents for a couple of months. If side effects are too severe, then start adding the killing agents very slowly.


The Food and Drug Administration has not evaluated these statements. None of the nutritional products mentioned are intended to diagnose, treat, cure, or prevent any disease.



Dr. Stephen Smith


Treating autistic children for over 15 years